Evolution of microbial aerosol behaviour in heating, ventilating and air-conditioning systems - quantification of Staphylococcus epidermidis and Penicillium oxalicum viability

Abstract : The aim of this study was to develop an experimental set-up and a methodology to uniformly contaminate several filter samples with high concentrations of cultivable bacteria and fungi. An experimental set-up allows contaminating simultaneously up to four filters for range of velocities representative of heating, ventilating and air-conditioning systems. The test aerosol was composed of a microbial consortium of one bacterium (Staphylococcus epidermidis) and one fungus (Penicillium oxalicum) and aerosol generation was performed in wet conditions. Firstly, the experimental set-up was validated in regards to homogeneity of the air flows. The bioaerosol was also characterized in terms of the number and particle size distribution using two particle counters: optical particle counter Grimm (R) 1.109 (optical diameters) and TSI APS 3321 (aerodynamic diameters). Moreover, stabilities of the number of particles generated were measured. Finally, concentrations of cultivable microorganisms were measured with BioSamplers SKC downstream of the four filters.
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Article dans une revue
Environmental Technology, Taylor & Francis: STM, Behavioural Science and Public Health Titles, 2012, 33 (19), pp.2217-2221. 〈10.1080/09593330.2012.689370〉
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https://hal-mines-nantes.archives-ouvertes.fr/hal-00877780
Contributeur : Nathalie Fontaine <>
Soumis le : mardi 29 octobre 2013 - 11:07:40
Dernière modification le : mercredi 11 juillet 2018 - 07:47:23

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Audrey Forthomme, Yves Andres, Aurélie Joubert, X. Simon, P. Duquenne, et al.. Evolution of microbial aerosol behaviour in heating, ventilating and air-conditioning systems - quantification of Staphylococcus epidermidis and Penicillium oxalicum viability. Environmental Technology, Taylor & Francis: STM, Behavioural Science and Public Health Titles, 2012, 33 (19), pp.2217-2221. 〈10.1080/09593330.2012.689370〉. 〈hal-00877780〉

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